How To's

How to Measure Airborne Microbial Contamination Using Passive Sampling or the Settling Plate Technique

A Comprehensive Literature Review & a Helpful Infographic

How To Use DIY Mould Test Kits

It's easy, just follow the steps

Infographic flowchart showing how to use the DIY mould testing kits

Of course, if you have any questions, just call or email for advice

You MUST use the kit within one (1) week of arrival. Petri plate nutrient media is like a foodstuff and will go off over time. In the event you do not use the kit promptly, you may need to re-order a new kit.


-Literature Review of the Method

Learn about how other people have used this method


  • Ejechi, B.O. and Ochei, O.P. (2017). Bacteriological safety assessment, hygienic habits and cross-contamination risks in a Nigerian urban sample of household kitchen environment. Environ Monit Assess. 189: 298. 
  • Vijayakumar, R., Saleh Al-Aboody, M., Alturaiki, W., Alsagaby, S.A. and Sandle, T. (2017). A study of airborne fungal allergens in sandstorm dust in Al-Zulfi, central region of Saudi Arabia. Journal of Environmental and Occupational Science. 6(1): 27-33. 
  • Wang, Y-H., Wang, Y-C., Wu, P-H., Hsu, L., Wang, C-Y., Jan, C-R. and Su, H-H. (2017). A cross-sectional study into the correlation of common household risk factors and allergic rhinitis in Taiwan's tropical environment. Asian Pacific Journal of Tropical Medicine. 10(2): 134-140. 
  • Molina-Veloso, A. and Borrego-Alonso, S.F. (2017). Viable allergenic fungi in a documentary deposit of the National Archive of Cuba. Revista Alergia Mexico. 64(1): 40-51.
  • Norbäck, D., Hisham Hashim, J.,  Cai, G-H., Hashim, Z., Ali, F., Bloom,E. and Larsson, L. (2016). Rhinitis, ocular, throat and dermal symptoms, headache and tiredness among students in schools from Johor Bahru, Malaysia: Associations with fungal DNA and mycotoxins in household dust. PLOS ONE. 1(11): 1-15
  • Luongo, J.C, Barberán, A, Hacker-Cary, R, Morgan, E.E., Miller, S.L. and Fierer, N. (2016). Microbial analyses of airborne dust collected from dormitory rooms predict the sex of occupants. Indoor Air. 28 March. Early View: 16th April 
  • Agodi, A. Auxilla, F., Barchitta, M., Cristina, M.L., D’Alessandro. D., Mura, I., Nobile, M., Pasquarella, C., Italian Study Group of Hospital Hygiene. (2015). Operating theatre ventilation systems and microbial air contamination in total joint replacement surgery: results of the GISIO-ISChIA study. J. Hospit Infect. 90(3): 213-219.
  • Fekadu, S. and Getachewu, B. (2015). Microbiological assessment of indoor air of teaching hospital wards: a case of Jimma University Specialised Hospital. Ethiopian Journal of Health Sciences. 25(2): 117-122.

  • Setlhare, G., Malebo, N., Shale, K. and Lues, R. (2014). Identification of airborne microbiota in selected areas in a health-care setting in South Africa. BMC Microbiology. 14: 100.

  • Napoli, C., Marcotrigiano, V. and Montagna, M.T. (2012). Air sampling procedures to evaluate microbial contamination: a comparison between active and passive methods in operating theatres. BMC Public Health. 12: 594.

  • Napoli, C. (2012). Prevention of healthcare-associated infections: which sampling method should be used to evaluate air bio-contamination in operating rooms? Epidemiology. 2:2.
  • Burr, H.N., Wolf, F.R. and Lipman, N.S. (2012). Corynebacterium bovis: Epizootiologic features and environmental contamination in an enzootically infected rodent room. Journal of the American Association for Laboratory Animal Science. 51(2): 189-198.

  • Brandys, R. C. and Brandys, G.M. (2011). Worldwide exposure standards for mold and bacteria - with assessment guidelines for air, water, dust, ductwork, carpet, insulation and bulk materials. 9th ed. Occupational and Environmental Health Consulting Services Inc, OEHCS


  • Bowling, F.L., Stickings, D.S., Edwards-Jones, V., Armstrong, D.G. and Boulton, A.J.M. (2009). Hydrodebridement of wounds: effectiveness in reducing would bacterial contamination and potential for air bacterial contamination. Journal of Foot and Ankle Research. 2: 13.

  • Khojasteh, V.J., Edwards-Jones, V., Childs, C. and Foster, H.A. (2007). Prevalence of toxin producing strains of Staphylococcus aureus in a pediatric burns unit. Burns. 33(3): 334-340. 

  • Andon, B.M. (2006). Active air vs. passive air (settle plate) monitoring in routine environmental monitoring programs. PDA J Pharm Sci Technol. 60(6): 350-355. 

  • Sandle, T. (2006). Environmental monitoring risk assessment. Journal of GXP Compliance. 10(2): 54-73.


  • ISO 14698-1:2003. Cleanrooms and associated controlled environments - biocontamination control - part 1: general principles and methods. [Note that this Standard remains current in 2015] and specifies the use of a "Suitable container (e.g. Petri dish) of appropriate size, containing an appropriate, sterile, culture medium which is left open for a defined period to collect viable particles depositing from the air. ISO 14698-1".
  • Pasquarella, C., Masia, M.D., Nnanga, N., Sansebastiano, G.E., Savino, A., Signorelli, C., Veronesi, L. (2004). Microbial air monitoring in operating theatre: active and passive samplings. Ann Ig. 16(1-2): 375-386. 

  • Pasquarella, C., Pitzurra, O. and Savino, A. (2000). The index of microbial air contamination. Journal of Hospital Infection. 46: 241-256. 

Contact form

Head Office: Level 1, 459 Toorak Rd, Toorak, VIC, 3142 Lab: 7/4 Weddel Court, Laverton North VIC, 3026, Australia Mon-Fri 8am - 6pm 1300132350